SynVivo是一種人工合成的、模擬體內(nèi)血管環(huán)境的微流體芯片，是用于模擬體內(nèi)實際液流和幾何學(xué)特征的體外模型。SynVivo由20個標準化的芯片組成，可模擬倉鼠、大鼠和小鼠不同血管的幾何學(xué)特征。芯片含有可培養(yǎng)細胞的腔隙，可用來研究細胞（如神經(jīng)元細胞，肝細胞和腫瘤細胞等）與**、循環(huán)分子間的相互作用。研究者只需將芯片的孔道覆蓋fibronectin類物質(zhì)來模擬體內(nèi)環(huán)境，進而在孔道上培養(yǎng)細胞，讓液體在上面流動模擬自然情況下體內(nèi)微血管網(wǎng)內(nèi)的血液，從而在近似體內(nèi)真實環(huán)境中研究所感興趣細胞的功能。
Microvascular Networks
Use Microvascular Networks to replicate in vivo cell/particle adhesion and cell-cell or cell-particle interactions in an in vitro setting. Investigate effects of flow and morphology for drug delivery, drug discovery and cellular behavior. Obtain shear-adhesion maps and bifurcation vs. branch adhesion in single experiment.
Microvascular Network Design Library
SMN1-C001 (104001)
SMN1-D001 (104002)
SMN1-C002 (104003)
SMN1-C003 (104004)
SMN1-C004 (104005)
SMN1-C005 (104006)
SMN1-C006 (104007)
SMN1-C007 (104008)
SMN1-D002 (104009)
SMN1-D003 (104010)
SMN1-D004 (104011)
SMN1-D005 (104012)
SMN1-D006 (104013)
SMN1-D007 (104014)
Use network co-culture assays to replicate the in vivo physiological and morphological conditions in addition to desired cellular makeup.
Co-Culture Network Design Library
By incorporating natural tissue regions within the network topology, the co-culture networks allow study of cell and drug behavior at and across the interfaces. The co-culture network constructs are available with several options for channel size, tissue region scaffolding, and barrier design. We can help you select the right parameters for your needs and can also construct custom designs if needed.
The SynVivo platform can support custom assays based on your research application. Don’t see a catalog assay for your biological question? Want to perform your assay using a linear chip design? We can develop a custom assay kit for you using our library of chip designs- See more information in the following tabs.
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Linear Channels
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Bifurcating Channels
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Microvascular Networks
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Idealized Networks
Custom Designs – If you need a specialized microvasulature or other design we have the necessary equipment to fabricate most any design based on your research needs. Our engineers will work with you to help design the most relevant SynVivo channel or network configuration to help you achieve your research goals.
Linear Channels
Use Linear Channels for studying cell/particle adhesion and cell-cell or cell-particle interactions at the micro-circulation scale. Use as a substitute for parallel plate flow chambers for >90% savings in consumables.
Linear Channels Design Library
Three channels per chip of various widths to allow you to study shear effects based on channel size and flow rates.
IMN1-LC
Standard Depth Options:
Standard Width Options (W1 / W2 / W3):
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100 μm / 100 μm / 100 μm
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250 μm / 250 μm / 250 μm
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500 μm / 500 μm / 500 μm
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100 μm / 250 μm / 500 μm
Custom designs/sizes also available.
Bifurcating Channels
Use symmetric and asymmetric bifurcations to study cell/particle adhesion and cell-cell or cell-particle interactions at bifurcations and to study the effect of the bifurcation angle and asymmetry on adhesion. Compare adhesion in linear sections and bifurcations simultaneously.
Bifurcating Channels Design Library
With various options of symmetric and asymmetric bifurcating angles and parent/daughter channel widths you can likely find a set of designs to provide you the best models for your research.
IMN1-BC
Standard Depth Options:
Standard Parent / Daughter Width (WA) / (WB + WC) Options:
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100 μm / 50 + 50 μm
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100 μm / 20 + 80 μm
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100 μm / 33 + 67 μm
Standard Symmetric Bifurcation Angle (θB/θC) Options:
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15° + 15°
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30° + 30°
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45° + 45°
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60° + 60°
Standard Asymmetric Bifurcation Angle (θB/θC) Options:
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6° + 24°
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10° + 20°
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15° + 75°
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18° + 72°
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30° + 60°
Custom designs/sizes also available.
Idealized Networks
Use idealized co-culture assays to mimic the cellular make up in vivo. Investigate cell-cell interactions and perfusion vs. diffusion based affects. Analyze the experiments in real-time for all the cell populations.
Co-Culture Design Library
Intended to mimic the formation of and transport across tight and gap junctions such as the blood-brain barrier and other endothelial/tissue interfaces, the idealized co-culture constructs are available with several options for channel size, tissue chamber size and scaffolding, and barrier design. We can help you select the right parameters for your needs and can also construct custom designs if needed. Please contact us for details.
Slit Barrier Option
This device utilizes slits and gaps to form the barrier region between the outer channel and inner chamber.
Standard design parameters available are:
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Outer Channel Width (OC): 100 μm or 200 μm
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Travel Width (T): 50 μm or 100 μm
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Slit Spacing (SS): 50 μm or 100 μm
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Slit Width (WS): Variable
IMN2/IMN3 (Slits)
MN2/IMN3 Chamber Zoom
IMN2/IMN3 Slit Details
應(yīng)用文獻
Publications
The following publications contain useful information about SynVivo technology and applications. Follow the doi links to see the abstracts and to obtain copies of the papers. Let us know if you have published a peer reviewed article utilzing SynVivo and we can add it to the list.
DOI:10.1371/JOURNAL.PONE.0142725
Adhesion Patterns in the Microvasculature are Dependent on Bifurcation Angle.G. Lamberti, F. Soroush, A. Smith, M. Kiani, B. Prabhakarpandian, K. Pant.Microvascular Res., 2015, 99, pp 19-25
DOI:10.1016/J.MVR.2015.02.004
Expanding Imaging Capabilities for Microfluidics: Applicability of Darkfield Internal Reflection Illumination (DIRI) to Observations in Microfluidics. Y. Kawano, C. Otsuka, J. Sanzo, C. Higgins, T. Nirei, T. Schilling, T. Ishikawa.PLoS ONE, 2015, 10(3): e0116925
DOI:10.1371/JOURNAL.PONE.0116925
Synthetic Tumor Networks for Screening Drug Delivery Systems. B. Prabhakarpandian, MC Shen, J. Nichols, C. Garson, I. Mills, M. Matar, J. Fewell, K. Pant. J Control Release., 2015, 201, 49-55
DOI:10.1016/J.JCONREL.2015.01.018
Bioinspired Microfluidic Assay for In Vitro Modeling of Leukocyte–Endothelium Interactions. G. Lamberti, B. Prabhakarpandian, C. Garson, A. Smith, K. Pant, B. Wang, and M.F. Kiani. Anal. Chem., 2014, 86 (16), pp 8344–8351
DOI:10.1021/AC5018716
Generation of Shear Adhesion Map Using SynVivo Synthetic Microvascular Networks. Smith, A. M., Prabhakarpandian, B., Pant, K. J. Vis. Exp. (87), e51025, 2014
DOI:10.3791/51025
Using shape effects to target antibody-coated nanoparticles to lung and brain endothelium. Kolhara P, Anselmob AC, Guptab V, Pant K, Prabhakarpandian B, Ruoslahtid E, and Mitragotri S. PNAS 2013
DOI:10.1073/PNAS.1308345110
Adhesive Interaction of Functionalized Particles and Endothelium in Idealized Microvascular Networks. G. Lamberti, Y. Tang, B. Prabhakarpandian, Y. Wang, K. Pant, M.F, Kiani, B. Wang. Microvascular Res. 2013 (89) pp 107-114
DOI:10.1016/J.MVR.2013.03.007
SyM-BBB: A Microfluidic Blood Brain Barrier Model B. Prabhakarpandian, M.-C. Shen, J.B. Nichols, I.R. Mills, M.S.-Wegrzynowicz, M. Aschner, K. Pant, Lab on a Chip, 2013, 13, 1093-1101
DOI:10.1039/C2LC41208J
Microfluidic devices for modeling cell-cell and particle-cell interactions in the microvasculature. Prabhakarpandian B, Shen MC, Pant K, Kiani MF. Microvasc Res. 2011 Nov;82(3):210-20
DOI:10.1016/J.MVR.2011.06.013
Bifurcations: focal points of particle adhesion in microvascular networks.Prabhakarpandian B, Wang Y, Rea-Ramsey A, Sundaram S, Kiani MF, Pant K.Microcirculation. 2011 Jul;18(5):380-9
DOI:10.1111/J.1549-8719.2011.00099.X
Flow and adhesion of drug carriers in blood vessels depend on their shape: a study using model synthetic microvascular networks. Doshi N, Prabhakarpandian B, Rea-Ramsey A, Pant K, Sundaram S, Mitragotri S. J Control Release. 2010 Sep 1;146(2):196-200
DOI:10.1016/J.JCONREL.2010.04.007
Preferential adhesion of leukocytes near bifurcations is endothelium independent. Tousi N, Wang B, Pant K, Kiani MF, Prabhakarpandian B. Microvasc Res. 2010 Dec;80(3):384-8
DOI:10.1016/J.MVR.2010.07.001
A physiologically realistic in vitro model of microvascular networks. Rosano JM, Tousi N, Scott RC, Krynska B, Rizzo V, Prabhakarpandian B, Pant K, Sundaram S, Kiani MF. Biomed Microdevices. 2009 May 19
DOI:10.1007/S10544-009-9322-8
Synthetic microvascular networks for quantitative analysis of particle adhesion. Prabhakarpandian B, Pant K, Scott RC, Patillo CB, Irimia D, Kiani MF, Sund